Cardiac diseases are considered the second most prevalent cause of death in dogs. Oxidative stress has been shown to induce damage in a variety of canine cardiovascular cell types and is implicated in the initiation and progression of various cardiac diseases.
Various antioxidant agents used in managing heart failure appear to confer cardio-protective benefits, but with growing evidence of the inadequacy of a monodimensional antioxidant strategy, there is a demand for an integrated approach using a combination of antioxidant compounds with complementary or interdependent effects. Specific data on the effects of natural antioxidant substances in canine endothelial cells are also lacking.
We developed a new canine aortic endothelial cell-based H202 oxidative stress assay. The antioxidant effects of four substances—L-carnitine, taurine, pomegranate extract and soy isoflavone extract, alone or in combination—were investigated with this new assay and two free radical scavenging assays.
Pomegranate extract, alone and in combination with the other substances, possesses significant strong antioxidant and cytoprotective activities in canine endothelial cells. Data from this and other published studies suggest that these natural substances could be of interest when developing a multidimensional dietary strategy to reduce the onset and progression of oxidative stress-induced canine disease and perhaps more specifically the endothelial degeneration involved in progressive valvular insufficiencies.
Source : C. Ripoll et al., 2012. Evaluation of natural substances’ protective effects against oxidative stress in a newly developed canine endothelial cell-based assay and in cell-free radical scavenging assays. Intern J Appl Res Vet Med 10: 113-124.