A new peer-reviewed study published in Microorganisms demonstrated that Benicaros (carrot rhamnogalacturonan-I) consistently modulates canine gut microbiome composition and metabolic activity across dogs of different sizes and breeds.
The research, conducted by NutriLeads in collaboration with dsm-firmenich, evaluated fecal samples from 18 healthy dogs representing small, medium and large size categories. The study found that the carrot-derived prebiotic significantly increased beneficial short-chain fatty acid production and selectively stimulated Phocaeicola vulgatus, a key SCFA-producing bacterial species.
Consistent effects across dog populations
Unlike traditional prebiotics that show variable responses, Benicaros delivered uniform results regardless of dog size, breed, or baseline microbiota composition. The ingredient consistently stimulated acetate and propionate production at low doses while reducing interindividual differences in microbiome response.
"This consistent production of SCFAs and the ability to balance the gut microbiota composition, suggests potential health benefits with Benicaros supplementation in dogs of all sizes," said Sue McKay, Ph.D., Nutrition & Health Manager at NutriLeads. "These findings highlight Benicaros' value as a next-generation precision prebiotic for companion animal health."
The study compared Benicaros against inulin, a commonly used prebiotic, and xanthan gum. While inulin showed variable responses across test subjects, Benicaros demonstrated more predictable effects. The ingredient selectively increased P. vulgatus levels, a bacterial species associated with gut barrier integrity and immune function benefits.
Formulation applications
Short-chain fatty acids serve as fuel for gut lining cells, strengthen barrier function, and help regulate immune responses. The consistent SCFA production observed with Benicaros supplementation supports digestive health and immune defense mechanisms in dogs.
"As a partner in this research, we were impressed by the robust and consistent effects of Benicaros on the canine gut microbiota of a broad target population," said Jeff Alix, global head of business development, Palatability & Microbiome at dsm-firmenich. "The ability of cRG-I to selectively enrich beneficial species while reducing variability across different dog sizes is promising.
"These findings not only reinforce the importance of precision prebiotics in dsm-firmenich's ambition to develop next-generation pet nutrition solutions but also exemplify our commitment to sustainability," Alix continued. "Benicaros is upcycled from carrot pomace — a valuable side stream of vegetable processing — which directly supports our strategic objective of revalorizing production side streams into high-impact, science-backed ingredients. Through this approach, we advance both pet health and environmental stewardship."
The ingredient's effectiveness at low daily doses offers formulation advantages for supplements, treats and functional pet foods. Benicaros is derived from carrot pomace, a byproduct of vegetable processing, supporting sustainability initiatives in ingredient sourcing.
"Benicaros will allow pet nutrition developers to formulate with confidence, knowing they're delivering consistent, science-backed benefits," said Joana Carneiro-Wakefield, Ph.D., CEO of NutriLeads.
The study was performed by ProDigest and represents continuing research into precision prebiotics for companion animal nutrition applications.
